Treating cutaneous squamous cell carcinoma using 5-aminolevulinic acid polylactic-co-glycolic acid nanoparticle-mediated photodynamic therapy in a mouse model
نویسندگان
چکیده
BACKGROUND Squamous cell carcinoma (SCC) is a common skin cancer, and its treatment is still difficult. The aim of this study was to evaluate the effectiveness of nanoparticle (NP)-assisted 5-aminolevulinic acid (ALA) delivery for topical photodynamic therapy (PDT) of cutaneous SCC. MATERIALS AND METHODS Ultraviolet-induced cutaneous SCCs were established in hairless mice. ALA-loaded polylactic-co-glycolic acid (PLGA) NPs were prepared and characterized. The kinetics of ALA PLGA NP-induced protoporphyrin IX fluorescence in SCCs, therapeutic efficacy of ALA NP-mediated PDT, and immune responses were examined. RESULTS PLGA NPs enhanced protoporphyrin IX production in SCC. ALA PLGA NP-mediated topical PDT was more effective than free ALA of the same concentration in treating cutaneous SCC. CONCLUSION PLGA NPs provide a promising strategy for delivering ALA in topical PDT of cutaneous SCC.
منابع مشابه
Synergistic Cytotoxic Effect of Gold Nanoparticles and 5-Aminolevulinic Acid-Mediated Photodynamic Therapy against Skin Cancer Cells
Background: Photodynamic therapy (PDT) is a promising therapeutic modality for the treatment of cancer and other diseases. In this study, the epidermoid carcinoma cell line A431 and the normal fibroblasts were used to investigate whether gold nanoparticles (GNPs) can induce an increase in cell death during PDT using 5-aminolevulinic acid (5-ALA) as a photosensitizer.Methods: Human fibroblast an...
متن کاملEffect of Silver Nanoparticles on Improving the Efficacy of 5-Aminolevulinic Acid-Induced Photodynamic Therapy
Introduction: The most important limitation of 5-aminolevulinic acid (5-ALA)-induced photodynamic therapy (PDT) is the efficacy of the cells in converting 5-ALA to protoporphyrin IX. The present study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) with the photosensitivity at the surface plasmon resonance wavelength on 5-ALA-mediated PDT. Material and Methods: First of a...
متن کاملEvaluation of the Primary Response of Basal Cell Carcinoma to Aminolevulinic Acid Photodynamic Therapy
Background: Basal Cell Carcinoma (BCC) is the most common type of skin cancer in human beings. Photodynamic therapy (PDT) is a novel therapeutic method which may be regarded as a non-invasive useful alternative for traditional treatments of BCC. This study was designed with the aim of evaluating the primary response of BCC to PDT.Methods: This clinical trial was perform...
متن کاملIn vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles
BACKGROUND 5-Aminolevulinic acid (ALA) is a prodrug for topical photodynamic therapy. The effectiveness of topical ALA can be limited by its bioavailability. The aim of this study was to develop a novel ALA delivery approach using poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs). METHODS A modified double emulsion solvent evaporation method was used to prepare ALA loaded PLGA NPs (ALA...
متن کاملEffects of Ultrasound Irradiation on the Release Profile of 5-fluorouracil from Magnetic Polylactic co-glycolic Acid Nanocapsules
Background: Drug nano-carriers are one of the most important tools for targeted cancer therapy so that undesired side effects of chemotherapy drugs are minimized. In this area, the use of ultrasound can be helpful in controlling drug release from nanoparticles to achieve higher treatment efficiency.Objective: Here, we studies the effects of ultrasound irradiation on the release profile of 5-flu...
متن کامل